General Analysis→Complete Analysis for any probe-target pair including reaction ΔG^o's, ΔG^ooverall, hybridization efficiency, and formamide dissociation profile.
ΔG^o_overall Series→ Calculate ΔG^o_overallvalues for a series of probes of constant length targeting a given region on the organism of interest
ΔG^o₁ Series→ Calculate ΔG^o₁values for a series of probes of constant length targeting a given region on the organism of interest
ΔG^o₂ Series→ Calculate ΔG^o₂values for a series of probes of constant length targeting a given region on the organism of interest
ΔG^o₃ Series→ Calculate ΔG^o₃values for a series of probes of constant length targeting a given region on the organism of interest
Mismatch Analysis→Evaluation of mismatch stability and its affect on formamide dissociation for given probe, perfect match target, and mismatched target triplet.
Formamide Curve Generator→Derivation of formamide dissociation profiles for multiple probe/target pairs.
Competitor Analysis→The effect of a competitor oligo on the formamide dissociation profile of the probe with both target and non-target.

ΔG^o_overall Series

WARNING : This calculator may take minutes to come up with the results.

Temperature : ^oC [ Na ⁺] : M

Enter the target frame :(?)Target frame is the fragment of the target sequence where probes of the specified length will be walked. The number of probes evaluated is calculated as (3′ end − 5′ end − probe length + 2). Ensure this is not greater than 20.

5' end: 3' end : Probe Length :

Select your Target Molecule :

Select the Domain of life for the Target molecule :

Enter the sequence of the Target Organism :(?)Accepts plain text, GenBank and Fasta Format.

ΔGooverall Series

ΔG^o_overall Series